ADP-ribosylation is the addition of one or moreADP-ribosemoieties to aprotein.[1][2] It is a reversiblepost-translational modification that is involved in many cellular processes, includingcell signaling,DNA repair,gene regulation andapoptosis.[3][4]Improper ADP-ribosylation has been implicated in some forms of cancer.[5] It is also the basis for the toxicity of bacterial compounds such ascholera toxin,diphtheria toxin, and others.[6]
The first suggestion of ADP-ribosylation surfaced during the early 1960s. At this time,Pierre Chambon and coworkers observed the incorporation ofATP into hen liver nuclei extract.[7] After extensive studies on the acid insoluble fraction, several different research laboratories were able to identifyADP-ribose, derived fromNAD+, as the incorporated group. Several years later, the enzymes responsible for this incorporation were identified and given the name poly(ADP-ribose)polymerase. Originally, this group was thought to be a linear sequence of ADP-ribose units covalently bonded through a ribose glycosidic bond. It was later reported that branching can occur every 20 to 30 ADP residues.[8]
The first appearance of mono(ADP-ribosyl)ation occurred a year later during a study of toxins: the diphtheria toxin ofCorynebacterium diphtheriae was shown to be dependent on NAD+ in order for it to be completely effective,[9] leading to the discovery of enzymatic conjugation of a single ADP-ribose group by mono(ADP-ribosyl)transferase.
It was initially thought that ADP-ribosylation was apost translational modification involved solely in gene regulation. However, as more enzymes with the ability to ADP-ribosylate proteins were discovered, the multifunctional nature of ADP-ribosylation became apparent. The first mammalian enzyme with poly(ADP-ribose)transferase activity was discovered during the late 1980s. For the next 15 years, it was thought to be the only enzyme capable of adding a chain of ADP-ribose in mammalian cells.[10] During the late 1980s, ADP-ribosyl cyclases, which catalyze the addition ofcyclic-ADP-ribose groups to proteins, were discovered. Finally,sirtuins, a family of enzymes that also possess NAD+-dependent deacylation activity, were discovered to also possess mono(ADP-ribosyl)transferase activity.[11][12]
The source of ADP-ribose for most enzymes that perform this modification is the redox cofactorNAD+. In this transfer reaction, theN-glycosidic bond of NAD+ that bridges the ADP-ribose molecule and the nicotinamide group is cleaved, followed bynucleophilic attack by the target amino acid side chain. (ADP-ribosyl)transferases can perform two types of modifications: mono(ADP-ribosyl)ation and poly(ADP-ribosyl)ation.
Mono(ADP-ribosyl)transferases commonly catalyze the addition ofADP-ribose toarginine side chains using a highly conserved R-S-EXE motif of the enzyme.[13] The reaction proceeds by breaking the bond betweennicotinamide and ribose to form anoxonium ion. Next, the arginine side chain of the target protein then acts a nucleophile, attacking the electrophilic carbon adjacent to the oxonium ion. In order for this step to occur, the arginine nucleophile isdeprotonated by aglutamate residue on the catalyzing enzyme[disputed –discuss]. Another conserved glutamate residue forms a hydrogen bond with one of the hydroxyl groups on the ribose chain to further facilitate this nucleophilic attack. As a result of the cleavage reaction, nicotinamide is released. The modification can be reversed by (ADP-ribosyl)hydrolases, which cleave theN-glycosidic bond between arginine and ribose to release ADP-ribose and unmodified protein; NAD+ is not restored by the reverse reaction.
Poly(ADP-ribose)polymerases (PARPs) are found mostly ineukaryotes and catalyze the transfer of multiple ADP-ribose molecules to target proteins. As with mono(ADP-ribosyl)ation, the source of ADP-ribose is NAD+. PARPs use acatalytic triad of His-Tyr-Glu to facilitate binding of NAD+ and positioning of the end of the existing poly(ADP-ribose) chain on the target protein; the Glu facilitates catalysis and formation of a (1''→2')O-glycosidic linkage between two ribose molecules.There are several other enzymes that recognize poly(ADP-ribose) chains,hydrolyse them or form branches; over 800 proteins have been annotated to contain the loosely defined poly(ADP-ribose) binding motif; therefore, in addition to this modification altering target protein conformation and structure, it may also be used as a tag to recruit other proteins or for regulation of the target protein.[14]
Many differentamino acidside chains have been described as ADP-ribose acceptors. From a chemical perspective, this modification represents proteinglycosylation: the transfer of ADP-ribose occurs onto amino acid side chains with a nucleophilic oxygen, nitrogen, or sulfur, resulting inN-,O-, orS-glycosidic linkage to the ribose of the ADP-ribose.[15] Originally, acidic amino acids (glutamate andaspartate) were described as the main sites of ADP-ribosylation. However, many other ADP-ribose acceptor sites such asserine,[16][17]arginine,[18]cysteine,[19]lysine,[20]diphthamide,[21]phosphoserine,[22] andasparagine[23] have been identified in subsequent works.
DuringDNA damage or cellular stress PARPs are activated, leading to an increase in the amount of poly(ADP-ribose) and a decrease in the amount of NAD+.[24] For over a decade it was thought that PARP1 was the only poly(ADP-ribose)polymerase in mammalian cells, therefore this enzyme has been the most studied.Caspases are a family of cysteineproteases that are known to play an essential role inprogrammed cell death. This protease cleaves PARP-1 into two fragments, leaving it completely inactive, to limit poly(ADP-ribose) production. One of its fragments migrates from the nucleus to the cytoplasm and is thought to become a target of autoimmunity.
During caspase-independentapoptosis, also called parthanatos, poly(ADP-ribose) accumulation can occur due to activation of PARPs or inactivation ofpoly(ADP-ribose)glycohydrolase, an enzyme thathydrolyses poly(ADP-ribose) to produce free ADP-ribose. Studies have shown poly(ADP-ribose) drives the translocation of the apoptosis inducing factor protein to the nucleus where it will mediateDNA fragmentation. It has been suggested that if a failure of caspase activation under stress conditions were to occur, necroptosis would take place. Overactivation of PARPs has led to anecrotic cell death regulated by thetumor necrosis factor protein. Though the mechanism is not yet understood, PARP inhibitors have been shown to affect necroptosis.[25]
ADP-ribosylation can affectgene expression at nearly every level of regulation, including chromatin organization, transcription factor recruitment and binding, and mRNA processing.
The organization ofnucleosomes is key to regulation of gene expression: the spacing and organization of nucleosomes changes what regions of DNA are available fortranscription machinery to bind and transcribe DNA.PARP1, a poly-ADP ribose polymerase, has been shown to affect chromatin structure and promote changes in the organization of nucleosomes through modification ofhistones.
PARPs have been shown to affecttranscription factor structure and cause recruitment of many transcription factors to form complexes at DNA and elicit transcription. Mono(ADP-ribosyl)transferases are also shown to affect transcription factor binding at promoters. For example, PARP14, a mono (ADP-ribosyl)transferase, has been shown to affectSTAT transcription factor binding.
Other (ADP-ribosyl)transferases have been shown to modify proteins that bindmRNA, which can causesilencing of that gene transcript.[26]
Poly(ADP-ribose)polymerases (PARPs) can function inDNA repair of single strand breaks as well as double strand breaks. In single-strand break repair (base excision repair) the PARP can either facilitate removal of an oxidized sugar or strand cleavage.PARP1 binds the single-strand breaks and pulls any nearby base excision repair intermediates close. These intermediates includeXRCC1 and APLF and they can be recruited directly or through the PBZ domain of the APLF.[27] This leads to the synthesis of poly(ADP-ribose). The PBZ domain is present in many proteins involved in DNA repair and allows for the binding of the PARP and thus ADP-ribosylation which recruits repair factors to interact at the break site.PARP2 is a secondary responder to DNA damage but serves to provide functional redundancy in DNA repair.[28]
There are many mechanisms for the repair of damaged double stranded DNA. PARP1 may function as asynapsis factor in alternative non-homologous end joining. Additionally, it has been proposed that PARP1 is required to slow replication forks following DNA damage and promoteshomologous recombination atreplication forks that may be dysfunctional. It is possible that PARP1 andPARP3 work together in repair of double-stranded DNA and it has been shown that PARP3 is critical for double-stranded break resolution. There are two hypotheses by which PARP1 and PARP3 coincide. The first hypothesis states that the two (ADP-ribosyl)transferases serve to function for each other's inactivity. If PARP3 is lost, this results in single-strand breaks, and thus the recruitment of PARP1. A second hypothesis suggests that the two enzyme work together; PARP3 catalyzes mono(ADP-ribosyl)ation and short poly(ADP-ribosyl)ation and serves to activate PARP1.[28]
The PARPs have many protein targets at the site of DNA damage.KU protein andDNA-PKcs are both double-stranded break repair components with unknown sites of ADP-ribosylation.Histones are another protein target of the PARPs. All core histones and linker histone H1 are ADP-ribosylated following DNA damage. The function of these modifications is still unknown, but it has been proposed that ADP-ribosylation modulates higher-orderchromatin structure in efforts to facilitate more accessible sites for repair factors to migrate to the DNA damage.
The ubiquitin-proteasome system (UPS) figures prominently in protein degradation. The26S proteasome consists of a catalytic subunit (the 20S core particle), and a regulatory subunit (the 19S cap).[29]Poly-ubiquitin chains tag proteins for degradation by the proteasome, which causes hydrolysis of tagged proteins into smaller peptides.
Physiologically, PI31 attacks 20S catalytic domain of 26S Proteasome that results in decreased proteasome activity. (ADP-ribosyl)transferase Tankyrase (TNKS) causes ADP-ribosylation of PI31 which in turn increases the proteasome activity. Inhibition of TNKs further shows the reduced 26S Proteasome assembly. Therefore, ADP-ribosylation promotes 26S Proteasome activity in bothDrosophila and human cells.[30]
The activity of someenzymes is regulated by ADP-ribosylation. For instance, the activity ofRodospirillum rubrumdi-nitrogenase-reductase is turned off by ADP-ribosylation of anarginine residue, and reactivated by the removal of the ADP-ribosyl group.[31]
PARP1 is involved inbase excision repair (BER), single- and double-strand break repair, and chromosomal stability. It is also involved intranscriptional regulation through its facilitation ofprotein–protein interactions. PARP1 usesNAD+ in order to perform its function in apoptosis. If a PARP becomes overactive the cell will have decreased levels of NAD+ cofactor as well as decreased levels ofATP and thus will undergonecrosis. This is important incarcinogenesis because it could lead to the selection of PARP1 deficient cells (but not depleted) due to their survival advantage during cancer growth.[32]
Susceptibility to carcinogenesis under PARP1 deficiency depends significantly on the type of DNA damage incurred. There are many implications that various PARPs are involved in preventing carcinogenesis. As stated previously, PARP1 and PARP2 are involved in BER and chromosomal stability. PARP3 is involved incentrosome regulation.Tankyrase is another (ADP-ribosyl)polymerase that is involved intelomere length regulation.[5]
PARP1 inhibition has also been widely studied in anticancer therapeutics. The mechanism of action of a PARP1 inhibitor is to enhance the damage done by chemotherapy on the cancerous DNA by disallowing the reparative function of PARP1 in BRCA1/2 deficient individuals .
PARP14 is another ADP-ribosylating enzyme that has been well-studied in regards to cancer therapy targets; it is a signal transducer and activator ofSTAT6 transcription-interacting protein, and was shown to be associated with the aggressiveness of B-cell lymphomas.[32]
Bacterial ADP-ribosylating exotoxins (bAREs) covalently transfer an ADP-ribose moiety of NAD+ to target proteins of infected eukaryotes, to yield nicotinamide and a free hydrogen ion. bAREs are produced asenzyme precursors, consisting of a "A" and "B" domains: the "A" domain is responsible for ADP-ribosylation activity; and, the "B" domain for translocation of the enzyme across the membrane of the cell. These domains can exist in concert in three forms: first, as single polypeptide chains with A and B domains covalently linked; second, in multi-protein complexes with A and B domains bound by non-covalent interactions; and, third, in multi-protein complexes with A and B domains not directly interacting, prior to processing.[6]
Upon activation, bAREs ADP-ribosylate any number of eukaryotic proteins; such mechanism is crucial to the instigation of the diseased states associated with ADP-ribosylation.GTP-binding proteins, in particular, are well-established in bAREs pathophysiology. For examples, cholera and heat-labile enterotoxin target theα-subunit of Gs ofheterotrimeric GTP-binding proteins. As the α-subunit is ADP-ribosylated, it is permanently in an "active", GTP-bound state; subsequent activation of intracellularcyclic AMP stimulates the release of fluid and ions from intestinal epithelial cells. Furthermore,C. BotulinumC3 ADP-ribosylates GTP-binding proteinsRho andRas, andPertussis toxin ADP-ribosylatesGi, Go, and Gt.Diphtheria toxin ADP-ribosylates ribosomal elongation factorEF-2, which attenuates protein synthesis.[6]
There are a variety of bacteria which employ bAREs in infection: CARDS toxin ofMycoplasma pneumoniae,cholera toxin ofVibrio cholerae;heat-labile enterotoxin ofE. coli;exotoxin A ofPseudomonas aeruginosa;pertussis toxin ofB. pertussis;C3 toxin ofC. botulinum; anddiphtheria toxin ofCorynebacterium diphtheriae.[33]