The1959–60 season was the 45th in the history of theIsthmian League, an Englishfootball competition.
At the end of the previous seasonRomford were transferred to theSouthern Football League, whileMaidstone United were newly admitted to the league fromAthenian League.
Tooting & Mitcham United were champions, winning their second Isthmian League title.
| Season | 1959–60 |
|---|---|
| Champions | Tooting & Mitcham United |
| Matches | 240 |
| Goals | 910 (3.79 per match) |
1960–61 → | |
| Pos | Team | Pld | W | D | L | GF | GA | GR | Pts |
|---|---|---|---|---|---|---|---|---|---|
| 1 | Tooting & Mitcham United | 30 | 17 | 8 | 5 | 75 | 43 | 1.744 | 42 |
| 2 | Wycombe Wanderers | 30 | 19 | 3 | 8 | 84 | 46 | 1.826 | 41 |
| 3 | Wimbledon | 30 | 18 | 3 | 9 | 66 | 36 | 1.833 | 39 |
| 4 | Kingstonian | 30 | 18 | 3 | 9 | 76 | 51 | 1.490 | 39 |
| 5 | Corinthian-Casuals | 30 | 18 | 1 | 11 | 69 | 61 | 1.131 | 37 |
| 6 | Bromley | 30 | 15 | 6 | 9 | 74 | 46 | 1.609 | 36 |
| 7 | Dulwich Hamlet | 30 | 14 | 6 | 10 | 65 | 47 | 1.383 | 34 |
| 8 | Walthamstow Avenue | 30 | 11 | 11 | 8 | 48 | 38 | 1.263 | 33 |
| 9 | Oxford City | 30 | 10 | 10 | 10 | 57 | 57 | 1.000 | 30 |
| 10 | Leytonstone | 30 | 10 | 8 | 12 | 43 | 46 | 0.935 | 28 |
| 11 | Woking | 30 | 10 | 6 | 14 | 54 | 61 | 0.885 | 26 |
| 12 | St Albans City | 30 | 10 | 6 | 14 | 50 | 65 | 0.769 | 26 |
| 13 | Maidstone United | 30 | 10 | 5 | 15 | 53 | 60 | 0.883 | 25 |
| 14 | Barking | 30 | 7 | 4 | 19 | 30 | 75 | 0.400 | 18 |
| 15 | Ilford | 30 | 5 | 6 | 19 | 34 | 86 | 0.395 | 16 |
| 16 | Clapton | 30 | 3 | 4 | 23 | 32 | 92 | 0.348 | 10 |