Class of enzymes
"GS-II" redirects here. For other, see
GS2 .
Protein family
1,3-Beta-glucan synthase is aglucosyltransferase enzyme involved in the generation ofbeta-glucan infungi . It serves as a pharmacological target forantifungal drugs such ascaspofungin ,anidulafungin , andmicafungin , deemed1,3-Beta-glucan synthase inhibitors . Under theCAZy classification system, fungi and plant members fall in theglycosyltransferase 48 family (GT48 ).[ 2] glycosyltransferase 2 family (Pfam PF13632 ), such as thecurdlan synthaseCrdS (Q9X2V0 ), also has a similar activity.[ 3]
Thebiosynthesis of disaccharides,oligosaccharides , andpolysaccharides involves the action of hundreds of differentglycosyltransferases . Theseenzymes catalyse the transfer ofsugar moieties from activated donormolecules to specific acceptor molecules, formingglycosidic bonds .
The family consists of various 1,3-beta-glucansynthase components including Gls1, Gls2, and Gls3 fromyeast . 1,3-Beta-glucansynthase (EC 2.4.1.34. ) also known as callose synthasecatalyses the formation of a beta-1,3-glucanpolymer that is a major component of thefungal cell wall .[ 4]
UDP-glucose + {(1,3)-beta-D-glucosyl}(N) = UDP + {(1,3)-beta-D-glucosyl}(N+1). ^a b c KFLGG,ENZYME: 2.4.1.34 ^ Campbell JA, Davies GJ, Bulone V, Henrissat B (September 1997)."A classification of nucleotide-diphospho-sugar glycosyltransferases based on amino acid sequence similarities" .Biochem. J .326 (3):929– 39.doi :10.1042/bj3260929u .PMC 1218753 PMID 9334165 . ^ Karnezis T, Epa VC, Stone BA, Stanisich VA (2003)."Topological characterization of an inner membrane (1→3)-beta-D-glucan (curdlan) synthase from Agrobacterium sp. strain ATCC31749" .Glycobiology .13 (10):693– 706.doi :10.1093/glycob/cwg093 PMID 12851288 . ^ Mio T, Adachi-Shimizu M, Tachibana Y, Tabuchi H, Inoue SB, Yabe T, Yamada-Okabe T, Arisawa M, Watanabe T, Yamada-Okabe H (July 1997)."Cloning of the Candida albicans homolog of Saccharomyces cerevisiae GSC1/FKS1 and its involvement in beta-1,3-glucan synthesis" .J. Bacteriol .179 (13):4096– 105.doi :10.1128/jb.179.13.4096-4105.1997 .PMC 179227 PMID 9209021 .
Activity Regulation Classification Kinetics Types
