Aviral vector is a modifiedvirus designed todeliver genetic material intocells. This process can be performed inside an organism or incell culture. Viral vectors have widespread applications in basic research, agriculture, and medicine.
Viruses have evolved specialized molecular mechanisms to transport theirgenomes into infected hosts, a process termedtransduction. This capability has been exploited for use as viral vectors, which may integrate their genetic cargo—thetransgene—into the host genome, although non-integrative vectors are also commonly used. In addition to agriculture and laboratory research, viral vectors are widely applied ingene therapy: as of 2022, all approved gene therapies were viral vector-based. Further, compared to traditionalvaccines, the intracellularantigen expression enabled byviral vector vaccines offers more robust immune activation.
Many types of viruses have been developed into viral vector platforms, ranging fromretroviruses tocytomegaloviruses. Different viral vector classes vary widely in strengths and limitations, suiting some to specific applications. For instance, relatively non-immunogenic and integrative vectors likelentiviral vectors are commonly employed for gene therapy. Chimeric viral vectors—such as hybrid vectors with qualities of bothbacteriophages and eukaryotic viruses—have also been developed.
Viral vectors were first created in 1972 byPaul Berg. Further development was temporarily halted by arecombinant DNA research moratorium following theAsilomar Conference and stringentNational Institutes of Health regulations. Once lifted, the 1980s saw both the first recombinant viral vector gene therapy and the first viral vector vaccine. Although the 1990s saw significant advances in viral vectors, clinical trials had a number of setbacks, culminating inJesse Gelsinger's death. However, in the 21st century, viral vectors experienced a resurgence and have been globally approved for the treatment of various diseases. They have been administered to billions of patients, notably during theCOVID-19 pandemic.
Viruses,infectious agents composed of a protein coat that encloses agenome, are the most numerous biological entities on Earth.[1][2] As they cannot replicate independently, they must infectcells and hijack thehost's replication machinery in orderto produce copies of themselves.[2] Viruses do this by inserting their genome—which can beDNA orRNA, eithersingle-stranded or double-stranded—into the host.[3] Some viruses mayintegrate their genome directly into that of the host in the form of aprovirus.[4]
This ability to transfer foreign genetic material has been exploited bygenetic engineers to create viral vectors, which cantransduce the desiredtransgene into a target cell.[2] Viral vectors consists of three components:[5][6]
Viral vectors are routinely used in abasic research setting and can introduce genes encoding, for instance,complementary DNA,short hairpin RNA, orCRISPR/Cas9 systems for gene editing.[8] Viral vectors are employed for cellular reprogramming, likeinducing pluripotent stem cells or differentiating adult somatic cells into different cell types.[9] Researchers also use viral vectors to createtransgenic mice and rats for experiments.[10] Viral vectors can be used forin vivo imaging via the introduction of areporter gene. Further, transduction of stem cells can permit the tracing of cell lineage duringdevelopment.[9]
Gene therapy seeks to modulate or otherwise affect gene expression via the introduction of a therapeutic transgene. Gene therapy by viral vectors can be performed byin vivo delivery by directly administering the vector to the patient, orex vivo by extracting cells from the patient, transducing them, and then reintroducing the modified cells into the patient.[11] Viral vector gene therapies may also be used for plants, tentatively enhancing crop performance or promoting sustainable production.[12]
There are four broad categories of gene therapy: gene replacement,gene silencing, gene addition, or gene editing.[11][13] Relative to other non-integrative gene therapy approaches, transgenes introduced by viral vectors offer multi-year long expression.[14]
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For use asvaccine platforms, viral vectors can be engineered to carry a specificantigen associated with an infectious disease or atumor antigen.[15][16] Conventional vaccines are not suitable for protection against some pathogens due to unique immune evasion strategies and differences in pathogenesis.[17] Viral vector-based vaccines, for instance, could eventually offer immunity againstHIV-1 andmalaria.[18]
While traditional subunit vaccines elicit a humoral response,[19] viral vectors allow for intracellular antigen expression that activates MHC pathways via both direct and crosspresentation pathways. This induces a robust adaptive immune response.[20][21] Viral vector vaccines also have intrinsicadjuvant properties via innate immune system activation and the expression ofpathogen-associated molecular patterns, negating the need for any additional adjuvant.[22][15] In addition to a more robust immune response in comparison to other vaccine types, viral vectors offer efficient gene transduction and can target specific cell types.[19] Pre-existing immunity to the virus used as the vector, however, can be a significant issue.[18]
Prior to 2020, viral vector vaccines were widely administered but confined to veterinary medicine.[22] In the global response to theCOVID-19 pandemic, viral vector vaccines played a fundamental role and were administered to billions of people, particularly in low and middle-income nations.[23]
Retroviruses—enveloped RNA viruses—are popular viral vector platforms due to their ability to integrate genetic material into the host genome.[2] Retroviral vectors comprise two general classes: gamma retroviral and lentiviral vectors. The fundamental difference between the two are that gamma retroviral vectors can only infect dividing cells, while lentiviral vectors can infect both dividing and resting cells.[24] Notably, retroviral genomes are composed of single-stranded RNA and must be converted to proviral double-stranded DNA, a process known asreverse transcription—before it is integrated into the host genome via viral proteins likeintegrase.[25]
The most commonly used gammaretroviral vector is a modifiedMoloney murine leukemia virus (MMLV), able to transduce various mammalian cell types. MMLV vectors have been associated with some cases of carcinogenesis.[26] Gammaretroviral vectors have been successfully applied toex vivo hematopoietic stem cell to treat multiple genetic diseases.[27]
Most lentiviral vectors are derived fromhuman immunodeficiency virus type 1 (HIV-1), although modifiedsimian immunodeficiency virus (SIV), thefeline immunodeficiency virus (FIV), and theequine infectious anaemia virus (EIAV) have also been utilized.[24] As all functional genes are removed or otherwise mutated, the vectors are notcytopathic and can be engineered to be non-integrative.[28]
Lentiviral vectors are able to carry up to 10 kb of foreign genetic material, although 3-4 kb was reported as optimal as of 2023.[24][28] Relative to other viral vectors, lentiviral vectors possess the greatest transduction capacity, due to the formation of a three-stranded "DNA flap" during retro-transcription of the single-strand lentiviral RNA to DNA within the host.[28]
Although largely non-inflammatory,[29] lentiviral vectors can induce robust adaptive immune responses by memory-typecytotoxic T cells andT helper cells.[30] This is largely due to lentiviral vectors' high tropism fordendritic cells, which activate T cells.[30] However, they can infect all types of antigen-presenting cells.[31] Moreover, as they are the only retroviral vectors able to efficiently transduce both dividing and non-dividing cells, make them the most promising vaccine platforms.[31] They have also been trialed as vaccines against cancer.[32]
Lentiviral vectors have been used asin vivo therapies, such as directly treating genetic diseases likehaemophilia B and forex vivo treatments like immune cell modification inCAR T cell therapy.[24] In 2017, theUS Food and Drug Administration (FDA) approvedtisagenlecleucel, a lentiviral vector, foracute lymphoblastic leukaemia.[33]
Adenoviruses are double-stranded DNA viruses belonging to the familyAdenoviridae.[34][35] Their relatively large genomes, of approximately 30–45 kb, make them ideal candidates for genetic delivery;[34] newer adenoviral vectors can carry up to 37 kb of foreign genetic material.[36] Adenoviral vectors display high transduction efficiency and transgene expression, and can infect both dividing and non-dividing cells.[37]
The adenoviral capsid, anicosahedron, features a fibre "knob" at each of its 12 vertices. These fibre proteins mediate cell entry—greatly affecting efficacy and contribute to its broad tropism—notably viacoxsackie–adenovirus receptors (CARs).[34][37] Adenoviral vectors can induce robust innate and adaptive immune responses.[38] Its strong immunogenicity is particularly due to the transduction of dendritic cells (DC), upregulating the expression of both MHC I and II molecules and activating the DCs.[39] They have a strong adjuvant effect, as they display severalpathogen-associated molecular patterns.[38] One disadvantage is that pre-existing immunity to adenovirus serotypes is common, reducing efficacy.[37][40] The use of chimpanzee adenoviruses may circumvent this issue.[41]
While the activation of both innate and adaptive immune responses is an obstacle for many therapeutic applications, it makes adenenoviral vectors an ideal vaccine platform.[35] The global response to the COVID-19 pandemic saw the development and use of multiple adenoviral vector vaccines, includingSputnik V, theOxford–AstraZeneca vaccine, and theJanssen vaccine.[42]
Adeno-associated viruses (AAVs) are relatively small single-stranded DNA viruses belonging toParvoviridae and, like lentiviral vectors, AAVs can infect both dividing and non-dividing cells.[43] AAVs, however, require the presence of a "helper virus" such as an adenovirus or herpes simplex virus to replicate within the host, although it can do so independently ifcellular stress is induced or the helper virus genes are carried by the vector.[44]
AAVs insert themselves into a specific site in the host genome, particularlyAAVS1 onchromosome 19 in humans. However, recombinant AAVs have been designed that do not integrate. These are instead stored as episomes that, in non-dividing cells, can last for years.[45] One disadvantage is that they are not able to carry large amounts of foreign genetic materials. Furthermore, the need to express the complementary strand for its single-stranded genome may delay transgene expression.[45]
As of 2020, 11 different AAV serotypes—differing by capsid structure and consequently by tropism—had been identified.[43] The tropism of adeno-associated viral vectors can be tailored by creating recombinant versions from multiple serotypes, termed pseudotyping.[43] Due to their ability to infect and induce longlasting effects within nondividing cells, AAVs are commonly used in basic neuroscience research.[46] Following the approval of the AAVAlipogene tiparvovec in Europe in 2012,[47] in 2017, the FDA approved the first AAV-based in vivo gene therapy—voretigene neparvovec—which treatedRPE65-associated Leber congenital amaurosis.[33] As of 2020, 230 clinical trials using AAV-based treatments were either underway or had been completed.[47]
Vaccinia virus, apoxvirus, is another promising candidate for viral vector development.[48] Its use as thesmallpox vaccine—first reported byEdward Jenner in 1798—led to the eradication ofsmallpox and demonstrated vaccinia as safe and effective in humans.[49][48] Moreover, manufacturing procedures developed to mass-produce smallpox vaccine stockpiles may expedite vaccinia viral vector production.[50]
Vaccinia possesses a large DNA genome and can consequently carry up to 40 kb of foreign DNA.[49][51][52][51] Further, vaccinia are unlikely to integrate into the host genome, decreasing the chance of carcinogenesis.[51] Attenuated strains—replicating and non-replicating—have been developed.[49] Although widely characterized due to its use against smallpox, as of 2019 the function of 50 percent of the vaccinia genome was unknown. This may lead to unpredictable effects.[52]
As a vaccine platform, vaccinia vectors display highly effective transgene expression and create a robust immune response.[50] The virus fast-acting: its life cycle produces mature progeny vaccinia within 6 hours, and has three viral spread mechanisms.[52] Vaccinia also has anadjuvant effect, activating a stronginnate response viatoll-like receptors.[50] A significant disadvantage that can reduce its efficacy, however, is pre-existing immunity against vaccinia in those who received the smallpox vaccine.[50]
Of the nineherpesviruses that infect humans, herpes simplex virus 1 (HSV-1) is the most well characterized and most commonly used as a viral vector.[53] HSV-1 offers several advantages: it has broad tropism and can deliver therapeutics via specialized expression systems.[54] Moreover, HSV-1 can cross the blood brain barrier if medically-disrupted, enabling it to target neurological diseases. Also, HSV-1 does not integrate into the host genome and can carry large amounts of foreign DNA. The former feature prevents harmful mutagenesis, as can occur with retroviral and adeno-associated vectors. Replication-deficient strains have been developed.[55]
In 2015,talimogene laherparepvec—an HSV-1 vector that triggers an anti-tumor immune response—was approved by the FDA to treatmelanoma.[56] As of 2020, HSV-1 vectors have been experimentally applied againstsarcomas and cancers of the brain, colon, prostate, and skin.[57]
Cytomegalovirus (CMV), a herpesvirus, has also been developed for use as a viral vector.[58] CMV can infect most cell types and can thus proliferate throughout the body. Although a CMV-based vaccine provided significant immunity against SIV—closely related to HIV—in macaques, development of CMV as a reliable vector was reported to still be in early stages as of 2020.[59][60]
Plant viruses are also engineered viral vectors for use in agriculture, horticulture, andbiologic production.[61] These vectors have been employed for a range of applications, from increasing the aesthetic quality ofornamental plants topest biocontrol, rapid expression of recombinant proteins and peptides, and to accelerate crop breeding.[62] The use of engineered plant viruses has been proposed to enhance crop performance and promote sustainable production.[12]
Replicating virus-based vectors are typically used.[63] RNA viruses used for monocots includewheat streak mosaic virus andbarley stripe mosaic virus and, for dicots,tobacco rattle virus. Single-stranded DNA viruses likegeminiviruses have also been utilized.[63] Viral vectors can be administered to plants via several pathways termed "agro-inoculation", including via rubbing, abiolistic delivery system, agrospray, agroinjection, and even viainsect vectors.[64][62] However,Agrobacterium-mediated delivery of viral vectors—in which bacteria are transformed withplasmid DNA encoding the viral vector construct—is the most common approach.[65]
Chimeric vectors combining both bacteriophages and eukaryotic viruses have been developed and are capable of infecting eukaryotic cells.[66][67] Unlike eukaryotic virus-based vectors, such bacteriophage vectors have no innate tropism for eukaryotic cells, allowing them to be engineered to be highly specific for cancer cells.[68]
Bacteriophage vectors are also commonly used in molecular biology.[69] For instance, bacteriophage vectors are used inphage-assisted continuous evolution, promoting rapid mutagenesis of bacteria.[70] Although limited tomycobacteriophages and some phages ofgram-negative bacteria, bacteriophages can be used for direct cloning.[71]
Viral vector manufacturing methods often vary by vector, although most utilize an adherent or suspension-based system with mammalian cells.[72] For viral vector production on a smaller, laboratory setting, static cell culture systems like Petri dishes are typically used.[73]
Those techniques used in the laboratory are difficult to scale, requiring different approaches on an industrial scale.[72] Large single-use disposable culture systems andbioreactors are commonly used by manufacturers.[72] Vessels such as those with gas permeable surfaces are used to maximize cell culture density and solution transducing units.[72] Depending on the vessel, viruses can be directly isolated from the supernatant or isolated via chemical lysis of the cultured cells or microfluidization.[74] In 2017,The New York Times reported a manufacturing backlog of inactivated viruses, delaying some gene therapy trials by years.[75]
In 1972,Stanford University biochemistPaul Berg developed the first viral vector, incorporating DNA from thelambda phage into the polyomavirusSV40 to infect kidney cells maintained in culture.[76][77][78] The implications of this achievement troubled scientists likeRobert Pollack, who convinced Berg not to transduce DNA from SV40 intoE. coli via a bacteriophage vector. They feared that introducing the purportedly cancer-causing genes of SV40 would create carcinogenic bacterial strains.[79][80] These concerns and others in the emerging field ofrecombinant DNA led to theAsilomar Conference of 1975, where attendees agreed to a voluntary moratorium oncloning DNA.[81]
In 1977, theNational Institutes of Health (NIH) issued formal guidelines confining viral DNA cloning to rigidBSL-4 conditions, practically preventing such research. However, the NIH loosened these rules in 1979, permittingBernard Moss to develop a viral vector utilizingvaccinia.[81] In 1982, Moss reported the first use of a viral vector for transient gene expression.[18] The following year, Moss used the vaccinia vector to express ahepatitis B antigen, creating the first viral vector vaccine.[22]
Every realm of medicine has its defining moment, often with a human face attached.Polio hadJonas Salk.In vitro fertilization hadLouise Brown, the world's first test-tube baby.Transplant surgery hadBarney Clark, the Seattle dentist with the artificial heart. AIDS hadMagic Johnson. Now gene therapy hasJesse Gelsinger.
Although a failed gene therapy attempt utilizing wild-typeShope papilloma virus had been made as early as 1972,Martin Cline attempted the first gene therapy utilizing recombinant DNA in 1980. It proved unsuccessful.[83][11] In the 1990s, as genetic diseases were further characterized and viral vector technology improved, there was overoptimism about the capabilities the technology. Many clinical trials proved failures.[84] There were some successes, such as the first effective gene therapy forsevere combined immunodeficiency (SCID); it employed a retroviral vector.[11]
However, during a 1999 clinical trial at theUniversity of Pennsylvania,Jesse Gelsinger died from a fatal reaction to an adenoviral vector-based gene therapy.[82][84] It was the first death related to any form of gene therapy.[85] Consequently, the FDA suspended all gene therapy trials at the University of Pennsylvania and investigated 60 others across the US.[85] An anonymous editorial inNature Medicine noted that it represented a "loss of innocence" for viral vectors.[84] Shortly thereafter, the field's reputation was further damaged when 5 children treated with a SCID gene therapy developedleukemia due to an issue with the retroviral vector.[84][note 1]
Viral vectors experienced a resurgence when they were successfully employed forex vivo hematopoietic gene delivery in clinical settings.[86] In 2003, China approved the first gene therapy for clinical use:Gendicine, an adenoviral vector encodingp53.[87][88] In 2012, the European Union issued its first approval of a gene therapy, an adeno-associated viral vector.[89] During theCOVID-19 pandemic, viral vector vaccines were used to an unprecedented extent: administered to billions of people.[90][22] As of 2022, all approved gene therapies were viral vector-based and over 1000 viral vector clinical trials targeting cancer were underway.[86]
In film, viral vectors are often portrayed as unintentionally causing a pandemic and civilizational catastrophe.[91] The 2007 filmI Am Legend depicts a cancer-targeting viral vector as unleashing azombie apocalypse.[92][93] Similarly, a viral vector therapy forAlzheimer's disease inRise of the Planet of the Apes (2011) becomes a deadly pathogen and causes anape uprising. Other films featuring viral vectors includeThe Bourne Legacy (2012) andResident Evil: The Final Chapter (2016).[94] An advanced form of viral vector vaccine is a critical story element inJurassic World Dominion (2022), in which it is used to cure a character's genetic disorder and later to stop a man-made ecological crisis.