Eukaryotic translation initiation factor 1A, X-chromosomal (eIF1A) is aprotein that in humans is encoded by theEIF1AXgene.[5][6][7] This gene encodes an essential eukaryotic translation initiation factor. The protein is a component of the 43S pre-initiation complex (PIC), which mediates the recruitment of the small40S ribosomal subunit to the5' cap of messenger RNAs.[7]
eIF1A is an important part of the translation intiation mechanism. It is located at the A-site of the small ribosomal subunit. During translation initiation, the 43S pre-initiation complex scans along the mRNA in search of a start codon. eIF1A's N-terminal tail interacts with the initiator tRNA and the start codon by extending into the P-site, thereby increasing the fidelity of start codon selection.[8] After the start codon has been selected andeIF1,eIF2, andeIF5 have left the pre-initiation complex,eIF5B is recruited to continue the initiation process. Here, eIF1A interacts with eIF5B such that eIF5B is remodeled into a conformation that allows joining of the large ribosomal subunit.[9] After the joining of the subunit, it is the dissociation of eIF1A that permits eIF5B to rearrange again, placing the tRNA in its final position.[9]
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Overview of all the structural information available in thePDB forUniProt:P47813 (Eukaryotic translation initiation factor 1A, X-chromosoma) at thePDBe-KB.
