Bacterial recombination is a type ofgenetic recombination inbacteria characterized byDNA transfer from one organism called donor to another organism as recipient. This process occurs in three main ways:
The final result of conjugation, transduction, and/or transformation is the production ofgenetic recombinants, individuals that carry not only the genes they inherited from their parent cells but also the genes introduced to their genomes by conjugation, transduction, and/or transformation.[5][6][7]
Recombination inbacteria is ordinarily catalyzed by aRecA type of recombinase.[8] These recombinases promoterepair ofDNA damages byhomologous recombination.[8]
The ability to undergo natural transformation is present in at least 67 bacterial species.[9]Natural transformation is common among pathogenic bacterial species.[10] In some cases, theDNA repair capability provided by recombination during transformation facilitates survival of the infecting bacterial pathogen.[10] Bacterial transformation is carried out by numerous interacting bacterialgene products.[9]
Evolution in bacteria was previously viewed as a result of mutation orgenetic drift.[11] Today, genetic exchange, or gene transfer is viewed as a major driving force in the evolution ofprokaryotes.[11] This driving force has been widely studied in organisms likeE. coli.[12] Bacteria reproduces asexually, where daughter cells are clones of the parent. This clonal nature leads to random mutations that occur during DNA replication that potentially helps bacteria evolve.[13] It was originally thought that only accumulated mutations helped bacteria evolve.[14] In contrast, bacteria also import genes in a process calledhomologous recombination, first discovered by the observation of mosaic genes at loci encoding antibiotic resistance.[11] The discovery of homologous recombination has made an impact on the understanding of bacterial evolution. The importance of evolution in bacterial recombination is its adaptivity. For example, bacterial recombination has been shown to promote the transfer of multi drug resistance genes via homologous recombination that goes beyond levels purely obtained by mutation.[15]
Bacterial recombination undergoes various different processes. The processes include: transformation, transduction, conjugation and homologous recombination. Homologous recombination relies on cDNA transferring genetic material.Complementary DNA sequences transport genetic material in the identical homologous chromosomes. The paternal and maternal paired chromosomes will align in order for the DNA sequences to undergo the process of crossing over.[16] Transformation involves the uptake of exogenous DNA from the encircling environment. DNA fragments from a degraded bacterium will transfer into the surrounding, competent bacterium resulting in an exchange of DNA from the recipient.[17] Transduction is associated with viral-mediated vectors transferring DNA material from one bacterium to another within the genome.[18] Bacterial DNA is placed into the bacteriophage genome via bacterial transduction. In bacterial conjugation, DNA is transferred via cell-to-cell communication. Cell-to-cell communication may involve plasmids that allow for the transfer of DNA into another neighboring cell.[19] The neighboring cells absorb the F-plasmid (fertility plasmid: inherited material that is present in the chromosome). The recipient and donor cell come into contact during a F-plasmid transfer. The cells undergohorizontal gene transfer in which the genetic material is transferred.[20]
Mechanisms for double-stranded breaks
The RecBCD pathway in homologous recombination repairs the double-strand breaks in DNA that has degraded in bacteria. Base pairs attached to the DNA strands go through an exchange at aHolliday junction. In the second step of bacterial recombination,branch migration. involves the base pairs of the homologous DNA strands to continuously be interchanged at a Holliday junction. This results in the formation of two DNA duplexes.[21] The RecBCD pathway undergoes helicase activity by unzipping the DNA duplex and stops when the nucleotide sequence reaches 5′-GCTGGTGG-3′. This nucleotide sequence is known as the Chi site. RecBCD enzymes will change after the nucleotide sequence reaches the Chi site. The RecF pathway repairs the degradation of the DNA strands.[18]
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