Secondary structure proposed for representative members of the familyAvsunviroidae
TheAvsunviroidae are a family ofviroids. There are five species in three genera (Avsunviroid,Elaviroid andPelamoviroid).[1] They consist of RNA genomes between 246 and 375nucleotides in length. They are single-stranded covalent circles and have intramolecular base pairing. All members lack a central conserved region.[2]
Replication occurs in thechloroplasts of plant cells. Key features of replication include no helper virus required and no proteins are encoded for. Unlike the other family ofviroids,Pospiviroidae,Avsunviroidae are thought to replicate via a symmetricalrolling mechanism. It is thought the positive RNA strand acts as a template to form negative strands with the help of an enzyme thought to beRNA polymerase plus 3 II. The negative RNA strands are then cleaved byribozyme activity and circularises. A second rolling circle mechanism forms a positive strand which is also cleaved by ribozyme activity and then ligated to become circular.The site of replication is unknown but it is thought to be in the chloroplast and in the presence of Mg2+ ions.[2]
Predictions of structure have suggested that they exist either as rod-shaped molecules with regions of base pairing causing formation of somehairpin loops or have branched configurations.[2][3]
The family has four stretches of conserved nucleotides,guuuc,uc,ucag,ac from 5' to 3', plus their Watson-Crick pairings on the other end of the loop. This is part of itshammerhead ribozyme.[1] Otherwise there is little structural similarity in the family.[Fl 1] They do not have the conserved CCH, TCR, or TCH motifs, which is one of the features defining their separation from the Pospiviroidae.[Fl 2]
The lack of a long, central conserved region makes Avsunviroidae harder to identify thanPospiviroidae. A method to detect them is to use their circularity: a computer can piece together many overlapping reads that appear to form repeats when placed linearly.[4]
^p.120, "Apart from the core nucleotides conserved in their hammerhead structures, no extensive sequence similarities exist between them"
^p.118-120, "The other four viroids,Avocado sunblotch viroid (ASBVd) (47),Peach latent mosaic viroid (PLMVd) (45),Chrysanthemum chlorotic mottle viroid (CChMVd) (65), andEggplant latent viroid (ELVd) (30), do not have the conserved CCR, TCR, and TCH motifs but, remarkably, both their polarity strands self-cleave through hammerhead ribozymes; they form the second family, Avsunviroidae (reviewed in 33), whose type species is ASBVd (formal inclusion of ELVd in this family is pending ICTV approval) (Figure 1b)."
^p.120, "ASBVd, the only viroid with a high A + U content (62%) (47), forms a monospecific genus"
^p.120, "and ELVd, whose properties fall between those of the members of the other two genera, has been proposed to constitute its own genus (30).
^p.120, "but PLMVd and CChMVd are grouped in one genus because of their branched secondary structure (21, 45, 65), which is stabilized by a pseudoknot (10; S. Gago, M. De la Peňa & R. Flores, unpublished results) (Figure 1b), and their insolubility in2M LiCl (65)."
