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dowser: B Cell Receptor Phylogenetics Toolkit

Provides a set of functions for inferring, visualizing, and analyzing B cell phylogenetic trees. Provides methods to 1) reconstruct unmutated ancestral sequences, 2) build B cell phylogenetic trees using multiple methods, 3) visualize trees with metadata at the tips, 4) reconstruct intermediate sequences, 5) detect biased ancestor-descendant relationships among metadata types Workflow examples available at documentation site (see URL). Citations: Hoehn et al (2022) <doi:10.1371/journal.pcbi.1009885>, Hoehn et al (2021) <doi:10.1101/2021.01.06.425648>.

Version:2.4.0
Depends:R (≥ 4.0),ggplot2 (≥ 3.4.0)
Imports:airr,alakazam (≥ 1.1.0),ape (≥ 5.6),Biostrings,dplyr (≥1.0),ggtree, graphics,gridExtra,markdown, methods,phangorn (≥ 2.7.1),phylotate,RColorBrewer,rlang,shazam (≥ 1.1.1), stats,stringr,tidyselect,tidyr, utils,treeio
Suggests:knitr,rmarkdown,testthat,pwalign,BiocManager
Published:2025-10-14
DOI:10.32614/CRAN.package.dowser
Author:Kenneth Hoehn [aut, cre], Cole Jensen [aut], Jessie Fielding [aut], Hunter Melton [aut], Susanna Marquez [ctb], Jason Vander Heiden [ctb], Steven Kleinstein [aut, cph]
Maintainer:Kenneth Hoehn <kenneth.b.hoehn at dartmouth.edu>
BugReports:https://github.com/immcantation/dowser/issues
License:AGPL-3
URL:https://dowser.readthedocs.io
NeedsCompilation:no
Citation:dowser citation info
Materials:README,NEWS
CRAN checks:dowser results

Documentation:

Reference manual:dowser.html ,dowser.pdf
Vignettes:Build Time Trees Using TyCHE and BEAST (source,R code)
Build Lineage Trees (source,R code)
Discrete Trait Analysis (source,R code)
Reconstruct Clonal Germlines (source,R code)
Measurable evolution (source,R code)
Using data from non-B cells (source,R code)
Plotting Lineage Trees (source,R code)
Quickstart (source,R code)
Building trees with paired heavy and light chain data (source,R code)
Reconstruct Intermediate Sequences (source,R code)

Downloads:

Package source: dowser_2.4.0.tar.gz
Windows binaries: r-devel:dowser_2.4.0.zip, r-release:dowser_2.4.0.zip, r-oldrel:dowser_2.4.0.zip
macOS binaries: r-release (arm64):dowser_2.4.0.tgz, r-oldrel (arm64):dowser_2.4.0.tgz, r-release (x86_64):dowser_2.4.0.tgz, r-oldrel (x86_64):dowser_2.3.tgz
Old sources: dowser archive

Linking:

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