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On March 20, 2013, CBS renewed CSI for a fourteenth season. The fourteenth season of CSI: Crime Scene Investigation will premiere on September 25, 2013, and contain 22 episodes.

CBS also confirmed that all current cast members will appear in Season 14. The series' 300th episode will air during this season, and Marg Helgenberger is set to guest star in the landmark episode. Due to the leave of absence taken by George Eads following his altercation with a writer, he will only appear in the first 4 episodes, under the pretext of receiving special training in Quantico. He will return later in the season.

The Devil and D.B. Russell also included a new redesigned Title Sequence.